An immunomagnetic cell separation system based on a retroviral vector containing a chimeric, recombinant human-murine CD4 gene

Primary B- and T-lymphocytes and neoplastic cells of B- and T-cell origin are particularly hard to transfect with plasmids.Thus, functional and molecular studies in immunology and oncohematology require selection of cells after plasmid transduction, assuring obtaining homogeneous cell populations expressing plasmid-delivered molecules.The selection

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Bicaudal D2, dynein, and kinesin-1 associate with nuclear pore complexes and regulate centrosome and nuclear positioning during mitotic entry.

BICD2 is one of the two mammalian homologues of the Drosophila Bicaudal D, an evolutionarily conserved adaptor between Refurbished Accessory microtubule motors and their cargo that was previously shown to link vesicles and mRNP complexes to the dynein motor.Here, we identified a G2-specific role for BICD2 in the relative positioning of the nucleus

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